Figure 1.

Determination of the efficiency of FXYD3 gene silencing in human colon adenocarcinoma (Caco-2) cells. (A) Western blot analysis of FXYD3 expression in wild-type or siRNA-treated Caco-2 cells after different time points in culture after confluency. Fifty micrograms of proteins of cell lysates were subjected to SDS-PAGE and transferred onto nitrocellulose membranes, and FXYD3 was immunodetected. After stripping, the nitrocellulose membrane was probed with a Na,K-ATPase α subunit antibody. lf, FXYD3 long form; sf, FXYD3 short form. (B) Quantification of short forms of FXYD3 proteins in Caco-2 cells after different time points in culture after confluency. Shown are arbitrary units obtained by densitometric scanning of data shown in A normalized by the amount of Na,K-ATPase α subunits. Shown are means ± SEM of four independent experiments. □, wild-type Caco-2 cells; ▩, siRNA1-treated Caco-2 cells; ■, siRNA2-treated Caco-2 cells.