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. 2009 Feb 15;20(4):1150–1166. doi: 10.1091/mbc.E08-06-0619

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© 2009 by The American Society for Cell Biology

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Figure 1. — Expansion of the Chlamydomonas centriole proteome reveals additional human disease genes. (A) Table of newly discovered POCs and BUGs. Version 3 gene identification numbers are as specified in version 3.0 of the Chlamydomonas genome sequence, available at the Joint Genomes Institute website at http://genome.jgi-psf.org/Chlre3/Chlre3.home.html. Table also indicates protein name, human Refseq ID numbers, localization of proteins to other proteomes of interest, GFP localization to centrioles in human cells, and any associated human diseases. (B) Localization of GFP-fusion proteins corresponding to human homologues of Chlamydomonas centriole candidate proteins (POC20/FAP124, BUG30/Ro/SSA, and BUG21/Mns1) after transient transfection into HeLa cells. γ-Tubulin antibody stain shows centrosomes; DAPI stain shows DNA. Each GFP-fusion protein colocalizes to a pair of dots within the centrosome in HeLa cells, confirming centriolar localization. Bar, 5 μm. (C) Venn diagrams illustrating the overlap of POC and BUG proteins with the Chlamydomonas flagellar proteome (Pazour et al., 2005) and with the Tetrahymena centriole proteome (Kilburn et al., 2007). Percentage of POC and BUG proteins overlapping with the specified proteomes are indicated.