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. 2009 Feb 15;20(4):1150–1166. doi: 10.1091/mbc.E08-06-0619

Figure 6.

Figure 6.

Immuno-EM of Chlamydomonas POC1 reveals localization to triplet microtubules and sites of fiber attachment. (A1–A4) Immuno-EM of basal body serial sections showing POC1 localization throughout the length of the triplet microtubules. (B1–B3) Serial sections showing POC1 localization to triplet microtubules and rootlet microtubules (root mt's). (C) POC1 localizes to doublet microtubules of axonemes but is absent from central pair microtubules. (D and E) POC1 localizes to rootlet microtubules that are nearby and/or attached to centrioles. (F–H) Sections demonstrating that POC1 localizes to proBBs and occasionally at the cartwheel (cartwheel indicated by black box). (I1–I5) Serial sections through a longitudinally sectioned basal body. POC1 localizes to sites of rootlet microtubule attachment and sites where proximal and distal connecting fibers attach to the basal body (root mt's, rootlet microtubules; pcf proximal connecting fiber, arrow shows a high density of POC1 at rootlet microtubule connection and at proximal connecting fiber). All sections were labeled with anti-POC1 antibody and gold-conjugated secondary antibodies. Bar, 250 nm.