Table 3.

Primers used for construct amplification of the IVS7 variant

Segment 1	Primer Sequence
IVS 5-3' BamHI	5'-GATGGGGATCCCAGCACCATCACACTGTTTGAG-3'
IVS6-5' XhoI	5'-CGGCGCTCGAGGAAATCATCACAAATGCCAAG-3'
Segment 2
IVS 6-3'	5'-CGCTCGAGGATGTTGTTCATGTAATAAA-3'
IVS 7-5' HindIII	5'-CGAAGCTTCCAACTGTGACAGCCCACAA-3'
Segment 3
IVS 7-3' HindIII	5'-GCAAGCTTGTGAGGCGTCATTAACTTGT-3'
IVS 8-5' PvuI	5'-GCCGATCGTTATTCCTGTTAGTTAGCAA-3'
Segment 4
IVS 8-3' PvuI	5'-GCCGATCGGCAGAGGGGTCTCAGAATCC-3'
IVS 9-5'	5'-CCTTCTGCCATTATCTCATTCTTG-3'