Figure 2.

Insulin secretion is higher in BLKS mice than B6 mice. (A) Glucose-stimulated insulin secretion was investigated in isolated islets from 12-week-old B6 and BLKS male mice. Following 48 h recovery post-isolation, the islets were stimulated with 2, 10 or 16.7 mM glucose for 1 h. Insulin release into the media was monitored. (B) Immunohistochemistry was performed in pancreatic islets from 26-week-old male mice revealing no gross differences in islet appearance or size. Insulin (oxidized DAB-brown) and glucagon (Vector Blue product) expressing cells are shown. (C) Pancreatic insulin content was assessed in pancreata excised from 15-week-old male B6 and BLKS mice (n = 3/group). No statistically significant differences were detected. All data represent means and s.e.m. and tested using a two-tailed Student's T-test assuming unequal variances; * P < 0.05; n.s. not significant.