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. Author manuscript; available in PMC: 2009 Dec 10.
Published in final edited form as: Respir Physiol Neurobiol. 2008 Dec 10;164(1-2):18–27. doi: 10.1016/j.resp.2008.07.024

Figure 2.

Figure 2

Example of techniques used to determine multiple transmitters in inspiratory-augmenting neurons of the rVRG. A. FluoroGold injection site in C4 cervical spinal cord. B. Neurons in rVRG retrogradely labeled with FG from injection site in A. C. Same neurons as in B labeled using in situ hybridization with an FITC-tagged riboprobe for pre-proenkephalin (ENK). D. Same neurons as in B labeled with a digoxigenin-tagged riboprobe fir VGlut2. E. BDA injection site in rVRG. F. Circled terminal in ventral horn of cervical spinal cord shows labels for BDA, VGlut2 and enkephalin revealed by immunocytochemistry. G. Inspiratory-augmenting neuron in rVRG labeled with Neurobiotin (NB). H. Same cell as G labeled with riboprobe for ENK mRNA. I. Inspiratory-augmenting neuron in rVRG labeled with Neurobiotin. J. Same cell as I labeled with riboprobe for VGlut2 mRNA. (Details in (Stornetta et al., 2003)).