(A) Primary MEFs generated from JAM-A+/+ and JAM-A−/− embryos were adsorbed with reovirus T1L, T3D, or T3SA- at MOIs of 0.01, 0.1, and 1 fluorescent focus unit (FFU)/cell and incubated for 20 hr. Reovirus antigen was detected by indirect immunofluorescence. Representative wells after adsorption with 1 FFU/cell are shown.
(B) Infected cells were quantified in five fields of 200X view for triplicate samples. Results are expressed as the mean FFU/field for triplicate experiments. Error bars indicate SD. *, P < 0.01 as determined by Student’s t test.
(C) Confluent monolayers of JAM-A+/+ and JAM-A−/− MEFs were adsorbed with reovirus T1L, T3D, or T3SA- at an MOI of 2 PFU/cell and incubated for the times shown. Viral titers were determined by plaque assay. Results are expressed as mean viral yields (tx/t0) for triplicate experiments. Error bars indicate SD.