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. Author manuscript; available in PMC: 2010 Jan 22.
Published in final edited form as: Cell Host Microbe. 2009 Jan 22;5(1):59–71. doi: 10.1016/j.chom.2008.12.001

Figure 1. JAM-A Is Required for Efficient Reovirus Infection of MEFs.

Figure 1

(A) Primary MEFs generated from JAM-A+/+ and JAM-A−/− embryos were adsorbed with reovirus T1L, T3D, or T3SA- at MOIs of 0.01, 0.1, and 1 fluorescent focus unit (FFU)/cell and incubated for 20 hr. Reovirus antigen was detected by indirect immunofluorescence. Representative wells after adsorption with 1 FFU/cell are shown.

(B) Infected cells were quantified in five fields of 200X view for triplicate samples. Results are expressed as the mean FFU/field for triplicate experiments. Error bars indicate SD. *, P < 0.01 as determined by Student’s t test.

(C) Confluent monolayers of JAM-A+/+ and JAM-A−/− MEFs were adsorbed with reovirus T1L, T3D, or T3SA- at an MOI of 2 PFU/cell and incubated for the times shown. Viral titers were determined by plaque assay. Results are expressed as mean viral yields (tx/t0) for triplicate experiments. Error bars indicate SD.