. Author manuscript; available in PMC: 2009 Feb 14.

Published in final edited form as: Autophagy. 2009 Feb 4;5(2):159–172. doi: 10.4161/auto.5.2.7328

Table 1A.

Enzymatic activity of recombinant ATG4.1 and ATG4.2 towards fluorogenic Abz-X-X-X-X-Q-EDDnp substrates (25 μM). Values are the means ± SE.

ATG8 homologue with sequence	Substrates	Specific Activities (nmoles.min⁻¹ mg protein⁻¹)
ATG8 homologue with sequence	Substrates	ATG4.1	ATG4.2
Hs-MAP-LC3	Abz-T-F-G-M-Q-EDDnp	99.4 ± 9.2	59.0 ± 3.2
ATG8	Abz-T-Y-G-G-Q-EDDnp	23.5 ± 7.7	<5
	Abz-T-Y-A-G-Q-EDDnp	111.6 ± 7.9	<5
ATG8A	Abz-S-M-G-A-Q-EDDnp	47.0 ± 9.2	<5
ATG8B	Abz-A-M-G-A-Q-EDDnp	311. 6 ± 8.2	144.5 ± 3.4
ATG8B	Abz-A-M-G-G-Q-EDDnp	28.1 ± 3.4	<5
	Abz-A-M-A-A-Q-EDDnp	149.7 ± 3.8	22.3 ± 3.5
ATG8B	Abz-I-A-G-L-Q-EDDnp	163.4 ± 4.2	83.7 ± 5.8
	Abz-I-A-A-L-Q-EDDnp	152.0 ± 6.2	83.5 ± 7.3
ATG8C	Abz-C-M-G-A-Q-EDDnp	128.9 ± 4.8	65.3 ± 8.4