Table 1B.

Inhibitory activity of peptidase inhibitors against recombinant ATG4.1 and ATG4.2. ATG4 (0.5 μg) was incubated with the inhibitors at room temperature for 15 min, and residual activity against 25 μM Abz-T-F-G-M-Q-EDDnp analysed. Values are the means ± SE.

Inhibitors	% Inhibition
	ATG4.1	ATG4.2
0.1 mM N-Ethylmaleimide (NEM)	99 ± 1.4	99 ± 1.9
1.0 mM Iodoacetamide	99 ± 2.1	97 ± 3.2
0.2 mM 1,10 phenanthroline	50 ± 9.3	43 ± 4.2
0.2 mM Phenylmethylsulfonyl fluoride (PMSF)	22 ± 8.4	17 ± 5.7
1.0μM E64	<1.0 ± 0.0	< 0.1 ± 0.0
1.0 mM Pepstatin A	<1.0 ± 0.0	<0.1 ± 0.0
0.5 mM EDTA	<1.0 ± 0.0	<0.1 ± 0.0