. Author manuscript; available in PMC: 2009 Feb 14.

Published in final edited form as: Autophagy. 2009 Feb 4;5(2):159–172. doi: 10.4161/auto.5.2.7328

Table 2.

Kinetics parameters of recombinant ATG4.1 activity towards Fluorescence Resonance Energy Transfer (FRET) peptides Abz-peptidyl-Q-EDDnp.

Substrate	K_m (μM)	k_cat (sec⁻¹)	kcat/K_m (μM⁻¹ sec⁻¹)
Abz-T-F-G-M-Q-EDDnp	0.6 ± 0.05	4.4 ±0.5	7.3
Abz-T-Y-G-G-Q-EDDnp	0.1 ± 0.05	1.1 ±0.4	10.7
Abz-T-Y-A-G-Q-EDDnp	0.6 ± 0.07	4.5 ±0.3	7.6
Abz-S-M-G-M-A-EDDnp	0.2 ± 0.05	1.6 ±0.5	7.8
Abz-A-M-G-A-Q-EDDnp	0.5 ± 0.05	8.8 ±0.0	17.7
Abz-A-M-G-G-Q-EDDnp	0.2 ± 0.06	1.4 ±0.2	6.6
Abz-A-M-A-A-Q-EDDnp	0.2 ± 0.07	5.7 ±0.3	28.4
Abz-I-A-G-L-Q-EDDnp	0.5 ± 0.04	4.9 ±0.3	9.9
Abz-I-A-A-L-Q-EDDnp	0.2 ± 0.09	5.4 ±0.2	26.8
Abz-C-M-G-A-Q-EDDnp	0.5 ± 0.08	6.2 ±0.3	12.3