Table 1.

Protein concentration of water-soluble crystallins isolated from wild type, αA-R49C heterozygous and αA-R49C homozygous mouse lenses^*.

Lens genotype	Protein concentration mg/ml
Wild type	6.0
αA-R49C heterozygous	4.8
αA-R49C homozygous	1.0

^*Lens water-soluble proteins were prepared by homogenizing mouse lenses in 1 ml Tris.Cl buffer pH 7.5 containing 100 mM NaCl, 1 mM DTT, 1 mM EDTA, 0.1%NaN₃ and centrifugation at 15,000 g as described in Materials and Methods. Four lenses were used for wild type and αA-R49C heterozygous mice. Because of the significantly smaller size of the lenses of the αA-R49C homozygous mice twelve lenses were used. Note the pronounced decrease in concentration of the soluble proteins in the αA-R49C homozygous lenses.