Fig. 3.

αGSU producing cells are misplaced in the double mutant. In the wildtype (A), Hes1 mutant (B), and Prop1 mutant (C) at e14.5 the αGSU immunoreactive cells are located exclusively in the anterior lobe as detected by immunohistochemistry on sagittal sections. However, in the double mutant, αGSU producing cells are detected within Rathke’s pouch (D, arrows). At e16.5 we continue to observe αGSU producing cells strictly within the anterior lobes of wildtype (E), Hes1 mutant (F), and Prop1 mutant (G) pituitaries while in the double mutant, αGSU producing cells appear to be located within Rathke’s pouch (H, arrow) in addition to the anterior lobe. n=3 Magnification: 200X