Fig. 5.

Loss of Hes1 and Prop1 does not affect initial stages of pituitary patterning. Sagittal sections of embryos at e12.5 were stained for expression of LHX3 (A-D) or ISL1 (E-H). LHX3 is detected throughout Rathke’s pouch and the anterior lobe in the wildtype (A), Hes11 mutant (B), Prop1 mutant (C), and double mutant (D). ISL1 expression is restricted to the anterior lobe in the ventral part of the pituitary in the wildtype (E, bracket), Hes1 mutant (F), Prop1 mutant (G), and double mutant (H). n=3 Magnification: 200X