Table 2.

Thermodynamic Parameters for the Interactions Shown in Figure 5

Figure	Interaction	N (stoichiometry)	Kd (nM)	ΔH (kcal. mol−1)	ΔS (cal. mol−1K−1)	C ([guide]/Kd)
5A	isolation, complementary	0.863 ± 0.0019	6.48 ± 0.62	−58.9 ± 0.24	−164	620
	isolation, mismatch	0.874 ± 0.0075	794 ± 30	−52.1 ± 0.66	−150	5.0
	+AfPiwi, complementary	0.919 ± 0.0044	14.2 ± 2.0	−38.6 ± 0.31	−95.7	280
	+AfPiwi, mismatch	1.146 ± 0.049	1160 ± 260	−29.6 ± 2.1	−73.8	3.5
5B	isolation, complementary (as Figure 3B)	0.811 ± 0.044	4600 ± 372	−47.3 ± 3.6	−137	1.1
	isolation, mismatch	0.80 (fixed)	14600 ± 840	−46.6 ± 1.3	−137	0.82
	+AfPiwi, complementary (as Figure 3B)	0.715 ± 0.0019	82.2 ± 2.7	−39.8 ± 0.15	−103	61
	+AfPiwi, mismatch	0.691 ± 0.0080	152 ± 16	−46.4 ± 0.74	−127	33
5C	isolation, complementary (as Figure 3B)	0.811 ± 0.044	4600 ± 372	−47.3 ± 3.6	−137	1.1
	isolation, mismatch	1.02 ± 0.19	6820 ± 1900	−37.0 ± 10	−102	0.73
	+AfPiwi, complementary (as Figure 3B)	0.715 ± 0.0019	82.2 ± 2.7	−39.8 ± 0.15	−103	61
	+AfPiwi, mismatch	0.764 ± 0.0053	92.9 ± 8.0	−43.9 ± 0.43	−117	54
5D	isolation, complementary	0.938 ± 0.0015	94.7 ± 2.1	−46.8 ± 0.12	−127	53
	isolation, mismatch	0.984 ± 0.0085	1280 ± 49	−44.0 ± 0.59	−123	3.9
	+AfPiwi, complementary	0.926 ± 0.0017	30.2 ± 1.3	−33.6 ± 0.11	−80.3	170
	+AfPiwi, mismatch	1.072 ± 0.019	2030 ± 150	−40.3 ± 1.30	−111	2.5
5E	isolation, complementary	1.11 ± 0.0040	990 ± 37	−50.6 ± 0.25	−145	24
	isolation, mismatch	1.14 ± 0.0046	7520 ± 150	−46.6 ± 0.32	−135	3.2
	+AfPiwi, complementary (as Figure 3A)	1.01 ± 0.0027	29.2 ± 2.0	−41.5 ± 0.18	−107	170
	+AfPiwi, mismatch	1.16 ± 0.0086	452 ± 27	−35.2 ± 0.38	−91.2	11
5F	isolation, complementary (as Figure 4D)	1.09 ± 0.0023	22.4 ± 1.4	−52.0 ± 0.18	−142	230
	isolation, mismatch	1.057 ± 0.0044	206 ± 7.9	−47.3 ± 0.27	−131	19
	+AfPiwi, complementary (as Figure 4D)	1.09 ± 0.0041	66.2 ± 4.0	−42.1 ± 0.24	−111	68
	+AfPiwi, mismatch	0.987 ± 0.022	672 ± 82	−36.8 ± 1.24	−97.2	6.0
5G	isolation, complementary (as Figure 4D)	0.996 ± 0.0041	25.4 ± 2.6	−56.5 ± 0.37	−158	200
	isolation, mismatch	1.059 ± 0.0062	1490 ± 40	−49.5 ± 0.47	−142	3.3
	+AfPiwi, complementary (as Figure 4D)	1.00 ± 0.0048	56.8 ± 4.8	−42.6 ± 0.31	−112	88
	+AfPiwi, mismatch	0.898 ± 0.046	2850 ± 350	−44.8 ± 3.5	−127	1.8
5H	isolation, complementary	1.11 ± 0.0040	990 ± 37	−50.6 ± 0.25	−145	24
	isolation, mismatch	1.07 ± 0.0021	926 ± 16	−49.5 ± 0.13	−141	18
	+AfPiwi, complementary (as Figure 3A)	1.01 ± 0.0027	29.2 ± 2.0	−41.5 ± 0.18	−107	170
	+AfPiwi, mismatch	1.07 ± 0.0021	17.0 ± 1.1	−37.6 ± 0.13	−92.7	290