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. 2009 Feb 23;4(2):e4563. doi: 10.1371/journal.pone.0004563

Figure 8. Validation of CLU and TGFBI apoptotic activity.

Figure 8

Panel A. Reduction of Clu and TGBFI proteins by gene specific siRNA knockdown assessed by Western blotting. YUMAC melanoma cells were untreated or treated with Aza (0.2 µM) for 2 days followed by transient transfection with siRNA directed to Clu, TGFBI, or a mixture of the two, employing Alexa Fluor as a control, as indicated. Cells were harvested the following day and extracts subjected to successive Western blotting with the respective antibodies, and anti-β-actin as a control. Panel B. Parallel cultures were tested for apoptosis with the Caspase 3/7 assay. Values are given as percent of control, i.e., non-transfected cultures.