Figure 9. Bortezomib Augments the Aza growth-arrest response in a synergistic manner.

Panel A. Dose-dependent effects of Bortezomib on cell proliferation. YUMAC melanoma cells were seeded in 24 well plates, incubated with increasing concentration of Bortezomib for 72 h, and cells from triplicate wells were harvested and counted with Coulter counter. Panels B and C. Growth responses of 501 mel and YURIF melanoma cells to 0.2 µM Aza (red), 2 nM Bortezomib (green) and combination (purple), compared to non-treated cells (black). Values are average of duplicate wells. Bars indicate double standard errors that ranged between 5–10% of total counts. Similar results were obtained with 1 nM Bortezomib in combination with Aza. The figures show one of two replicate experiments with similar results.