Figure 4. Administration of GSH diminishes ROS levels and enhances survival within wild-type colonies, as well as during seeding on highly respiratory media.

(A) Approximately 100 colonies were grown for 36 hours on SCGlu plates with or without 5 mM GSH, washed off and clonogenic assays were performed with the collected cells (mean±SEM, n = 3; **p<0.01). Additionally equal amounts of cells were stained for reactive oxygen species (ROS) with dihydroethidium (DHE) and quantified using a fluorescence microplate reader (Genios-Pro). Values of relative fluorescent units (RFU) are displayed (mean±SEM, n = 3; **p<0.01) (B). (C) Clonogenic assay of cultures pre-grown in liquid SCGlu. 500 cells were plated on SCGlu, SCGal, or SCGly agar plates, respectively, with or without 1 mM GSH (mean±SEM, n = 2; ***p<0.001).