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. Author manuscript; available in PMC: 2009 Oct 14.
Published in final edited form as: Mutat Res. 2008 Aug 5;645(1-2):9–18. doi: 10.1016/j.mrfmmm.2008.07.013

Figure 1. Arrangement of chromosome III and chromosome V markers in diploid yeast strains used in the study.

Figure 1

A. Diploid strains AM960 and AM993. A DSB (black vertical arrow) is induced at MATa by a galactose-inducible HO gene. HML and HMR are replaced by ADE1 in the MATa-containing chromosome (Chr) III. URA3 is inserted 133 kb from the left telomere, approximately 67 kb proximal to MATa. The other Chr III homologue contains MAT α-inc that cannot be cut by HO, has a mutant copy of thr4. FS2 consists of two Ty1 elements (labeled Ty1α and Ty1β) in inverted orientation [27] and located 30 kb proximal to MAT. FS1 consists of two Ty1 elements in direct orientation located 57 kb centromere-proximal to MATa. B. Diploid strains AM1111 and AM1112. The chromosomal arrangements in these strains are similar to those shown in A, but with an inverted repeat of FEN2 replacing FS2 in the MATa-containing chromosome. The distance between the two inverted copies of FEN2 is 1 kb. Also, HMR in this strain is replaced by NAT. In addition, one copy of Chr V contains a copy of FEN2 inserted at YER186C located approximately 13 kb from the telomere. C. The expected structure of chromosome III in various outcomes resulting from repair of HO-induced DSBs in strains shown in B. Repair outcomes for strains shown in A are identical with the exception that HMRa remains intact.