Table 2.
The effect of IRs of Ty1 on DSB repair in rad51Δ and rad54Δ diploids.
| Strain | IRa | No. Colonies tested | % Event for phenotype of coloniesb |
Viability YEP-Gal (%) | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Ade+Thr+ | Ade+Thr− | Ade+/−Thr− | Ade−Thr | |||||||
| or Ade+Thr+/− | Ura+ | Ura+/− | Ura− | Ura+/− | Ura− | |||||
|
RAD51c (YLS100) |
FS2 | 638 | 98.7 | 1.0 | 0.3 | 0.0 | 93 ± 6 | |||
|
rad51Δ (AM960) |
FS2 | 1304 | 2.2 | 7.5 | 6.3 | 4.5 | 56.9 | 9.5 | 13.1 | 92 ± 12 |
|
rad51Δ (AM990) |
Ty1αΔ Ty1βΔ |
2038 | 0.5 | 2.4 | 7.4 | 10.0 | 14.4 | 24.1 | 41.2* | 93 ± 13 |
|
rad51Δ (AM991) |
Ty1αΔ Ty1βΔ |
402 | 0.0 | 8.2 | 2.2 | 10.2 | 17.0 | 17.0 | 45.4* | 88 ± 4 |
|
rad51Δ (AM992) |
Ty1αΔ Ty1βΔ (ars310-Δ1) |
668 | 0.6 | 3.4 | 2.4 | 10.2 | 17.8 | 23.7 | 41.9* | 82 ± 6 |
|
rad54Δ (AM993) |
FS2 | 550 | 1.5 | 2.9 | 0 | 4.9 | 16.4 | 22.7 | 51.6* | 84 ± 9 |
a
Indicates inverted DNA repeats present in the MATa –containing copy of chromosome III undergoing DSB repair.
b
Some colonies were sectored for one or more nutritional markers. A colony that was half Ade+ and half Ade- is indicated as Ade+/−
c
Data for RAD51 diploid YLS100 (except for viability) are taken from Signon et. al. [24]. The viability for this diploid was determined in this study.
*
Indicates a statistically significant difference from the isogenic rad51Δ diploid strain with intact FS2 region (P<0.001).