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. Author manuscript; available in PMC: 2009 Dec 12.
Published in final edited form as: Biochem Biophys Res Commun. 2008 Oct 16;377(2):658–661. doi: 10.1016/j.bbrc.2008.10.035

Figure 2.

Figure 2

Evidence for promoter-specific recruitment of Mam. In the absence of bHLH A proteins, co-expression of NICD with either wild-type Mam (MamWT) or a MamΔC-VP16 fusion protein activated the m8 and mγ promoters. By contrast, co-expression of these proteins did not activate the SPS-4A or ac-SPS promoters. The ability of all these promoters to mediate Notch-proneural transcriptional synergy indicated that functional Su(H)/NICD complexes can form on the respective SPS elements of these promoters (Figure 1). However, the promoter-specific co-activation by Mam in the absence of bHLH A proteins suggests that recruitment of Mam and the formation of functional Su(H)/NICD/Mam ternary complexes occurs on the native m8 and mγ promoters. Diagrams of the Mam proteins expressed are shown in the insert. The region within Mam required for binding both Su(H) and NICD is indicated by the shaded box.