(A and B) Schematic diagrams of the focal electroporation experiment and the Nkx2-1 (372 amino acids) constructs used in these experiments.
(C–G) Migration of MGE-derived cells electroporated with Gfp (C) or with Gfp and Nkx2-1 (D), Nkx2-1ΔTN (E), Nkx2-1ΔCt (F) or Nkx2-1A35T (G). Arrowheads point to cells that have reached the cortex. Dotted lines indicate the limits of the organotypic slices.
(H) Schematic representation of the migratory routes adopted by MGE-derived cells electroporated with Gfp and Gfp + Nkx2-1A35T (green arrow) or with Gfp Nkx2-1, Nkx2-1ΔTN or Nkx2-1ΔCt (black dotted arrow).
GP, globus pallidus; H, hippocampus; HD, homeodomain; LGE, lateral ganglionic eminence; MGE, medial ganglionic eminence; NCx, neocortex; NK2-SD, NK2 specific domain; PCx, piriform cortex; POA, preoptic area; Str, striatum; TN, Tinman motif.
Scale bar equals 200 µm.