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. Author manuscript; available in PMC: 2009 Nov 25.
Published in final edited form as: Virology. 2008 Oct 2;381(2):194–202. doi: 10.1016/j.virol.2008.08.027

Fig. 3.

Fig. 3

DNA slot blot analysis for AAV2 entry into HeLa cells after in vitro phosphorylation of AAV2 capsids by EGFR-PTK. HeLa cells were mock-infected (lane 1) or infected with AAV2-lacZ vectors, which were either mock-incubated (lane 2) or pre-incubated with ATP (lane 3), EGFR-TPK (lane 4) or both (lane 5). Following digestion with trypsin to degrade adsorbed and unadsorbed vectors, low-Mr DNA samples were isolated 2 hrs post-infection and analyzed on by slot blot hybridization using a 32P-labeled lacZ DNA probe.