Figure 2.

Stabilization of urokinase-type plasminogen activator receptor (uPAR) mRNA by LPS. Beas2B cells were incubated with phosphate-buffered saline (PBS) or LPS for 12 hours to induce maximum expression of uPAR mRNA. Ongoing transcription was later blocked by adding 5, 6-dichloro-1-β-D-ribofuranosyl benzamidazole (DRB) (20 μg/ml) to the same media. Total RNA was isolated and uPAR mRNA level was measured at predefined time points after addition of DRB by Northern blot using a ³²P-labeled uPAR cDNA probe, followed by hybridization with a β-actin probe to assess for equal loading. The line graph represents the percentage of mRNA decay relative to Time 0 (designated 100%), calculated from the mean values obtained by integrating the densities of individual bands from two independent experiments.