Figure 4.

Effect of LPS on heterogeneous nuclear ribonucleoprotein C (hnRNPC) and urokinase-type plasminogen activator receptor (uPAR) mRNA 3′ UTR binding in Beas2B cells. (A) Beas2B cells grown in culture plates were incubated with phosphate-buffered saline or LPS for 0 and 24 hours, as described in Figure 3. hnRNPC proteins were isolated from these cell lysates, and processed as described above. The line graph represents the mean and SD from three independent experiments. (B) Beas2B cells grown in culture plates were incubated with LPS for 0–24 hours, as described above. hnRNPC proteins were isolated from these cell lysates, separated by sodium dodecyl sulfate (SDS)–polyacrylamide gel electrophoresis (PAGE), and transferred to nitrocellulose membranes. The membranes were later incubated with [³²P]uPAR mRNA 3′ untranslated region. Western blot analysis using anti-hnRNPC antibody was performed on the same membranes after stripping. The line graph represents the mean and SD from three independent experiments.