Figure 5.

Role of urokinase-type plasminogen activator (uPA) in LPS-induced uPA receptor (uPAR) expression in mouse lungs. (A) Membrane proteins (100 μg/lane) isolated from lung homogenates of phosphate-buffered saline or LPS-challenged wild-type (WT) or uPA^−/− mice were separated by sodium dodecyl sulfate (SDS)–polyacrylamide gel electrophoresis (PAGE) under nonreducing conditions and transferred to nitrocellulose membranes. The membranes were immunoblotted with anti-uPAR antibody. The same membranes were later stripped and developed with β-actin antibody for equal loading. The bar graph shows the results (mean + SD) from three independent experiments. (B) Total RNA (20 μg) isolated from lung homogenates of the above-treated mice was separated on 1% agarose/formaldehyde gels and subjected to Northern blotting using ³²P-labeled uPAR cDNA and β-actin cDNA probes. The results shown are representative of three independent experiments.