Figure 6.

The role of urokinase-type plasminogen activator (uPA) in LPS-induced uPA receptor (uPAR) expression by alveolar epithelial cells isolated from mouse lungs. Membrane proteins (100 μg/lane) extracted from alveolar epithelial cells of phosphate-buffered saline or LPS-challenged WT or uPA^−/− mice were separated by sodium dodecyl sulfate (SDS)–polyacrylamide gel electrophoresis (PAGE) under nonreducing conditions. The resolved proteins were then electrotransferred to nitrocellulose membranes and immunoblotted with anti-uPAR antibody. The same membranes were then stripped and developed with β-actin antibody to confirm equal loading. The bar graph shows the results (mean + SD) from three independent experiments.