Figure 8.

Effect of LPS on phosphoglycerate kinase (PGK) phosphorylation and its interaction with urokinase-type plasminogen activator receptor (uPAR) mRNA coding region (CDR). PGK isolated from lung homogenates of WT and uPA^−/− mice challenged with phosphate-buffered saline (PBS) or LPS, as described in Figure 6, were separated on 8% sodium dodecyl sulfate–polyacrylamide gels under nonreducing conditions. PGK was transferred to nitrocellulose membranes. The membranes were analyzed for PGK-uPAR mRNA CDR interaction by Northwestern assay using a ³²P-labeled uPAR mRNA CDR probe. The same membranes were later stripped and analyzed for tyrosine phosphorylation and total PGK expression by Western blotting using anti-phosphotyrosine and anti-PGK antibodies, respectively. The bar graph shows mean and (+ SD) densitometric values from three independent experiments.