Figure 9.

The role of LPS on hnRNPC binding to urokinase-type plasminogen activator receptor (uPAR) mRNA 3′ untranslated region (UTR) and hnRNPC tyrosine phosphorylation. hnRNPC protein isolated from crude lung extracts of WT and uPA^−/− mice challenged with phosphate-buffered saline or LPS were separated by sodium dodecyl sulfate (SDS)–polyacrylamide gel electrophoresis (PAGE) under nonreducing conditions and transferred to nitrocellulose membranes. The membranes were later analyzed for uPAR 3′ UTR mRNA interaction by Northwestern assay using a ³²P-labeled uPAR mRNA 3′ UTR probe. The same membranes were stripped and analyzed for tyrosine phosphorylation and total hnRNPC expression by Western blotting using anti-phosphotyrosine and anti-hnRNPC antibodies, respectively. The bar graph represents the data (mean + SD) from three independent experiments.