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pH-rate profile of E222Q maturation, demonstrating the dependence of the observed rate constant on pH. Blue (-●-), unlabeled protein; Red (-■-), Cβ-di-deuterotyrosine-labeled protein. The symbols represent experimental data, and the solid lines represent curve fits to kinetic equations (see Methods). Inset: Increase in chromophore absorbance (483 nm) over time, measured at pH 8.
