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. 2009 Feb;30(1):3–17.

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The contents of articles or advertisements in The Clinical Biochemist – Reviews are not to be construed as official statements, evaluations or endorsements by the AACB, its official bodies or its agents. Statements of opinion in AACB publications are those of the contributors. Print Post Approved - PP255003/01665. Copyright © 2005 The Australasian Association of Clinical Biochemists Inc. No literary matter in The Clinical Biochemist – Reviews is to be reproduced, stored in a retrieval system or transmitted in any form by electronic or mechanical means, photocopying or recording, without permission. Requests to do so should be addressed to the Editor. ISSN 0159 – 8090

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Separation of five biogenic amines (50 pmol each on-column) on an Atlantis T3 column (150 x 2.1 mm, 3 μm particle size) at 25°C with a flow rate of 200 μL/min of 0.2% formic acid-methanol (98/2, v/v). Peaks were detected by tandem mass spectrometry with multiple reaction monitoring of the ion transition pairs as follows: (1) noradrenaline 170→152, (2) adrenaline 184→166, (3) normetanephrine 166→134, (4) dopamine 154→137 and (5) metanephrine 180→148. The smaller normetanephrine peak is due to the 184→166 ion pair which is common with adrenaline.