Fig. 4.

BHst 5 binds with epitopes in Ssa1 and 2 peptide arrays. BHst 5 (2 μM) was incubated with Ssa1 or Ssa2 peptide array membranes for 3 h at room temperature. After extensive washing with TBS buffer to remove unbound BHst 5, the membrane was incubated with Streptavidin-HRP (1:20 000) for 1 h at room temperature and the image developed with SuperSignal West Pico Chemiluminescence Substrate. Hst 5 binding to peptides sequences unique to Ssa2 protein is shown.