Figure 4.

Inhibitory effects of 100 μM La³⁺, 100 μM Gd³⁺, and 50 μM 2-aminoethoxydiphenylborane (2-APB) on CPA-induced inward currents in single HASM cells assessed by whole cell patch clamp. (A) Time course of current density (inward currents measured at −80 mV, outward current measured at +80 mV) showing control cells versus cells treated with 100 μM La³⁺, each point represents mean data ± SEM of all cells in each group; control cells (n = 9), La³⁺-treated cells (n = 9). (Bi) Representative current–voltage (I-V) relationships at point 1 from the time course. (Bii) A bar chart illustrating CPA-sensitive inward current density at point 1 (measured at −80 mV) of control cells compared with cells treated with 100 μM La³⁺, 100 μM Gd³⁺, and 50 μM 2-APB. Data are indicated as statistically significant with *P < 0.05, **P < 0.01.