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Thyroid transcription factor (TTF)-1 and pro–surfactant protein-C (pSPC) immunostaining of immortal (A, C) and AT2 epithelial cells (B, D). Cells were incubated with an antibody to TTF-1 (A, B) or pSPC (C, D). AT2 cells were positive for TTF-1 (B, nuclear stain) and for pSPC (D), whereas immortal cells were negative for both TTF-1 (A) and pSPC (C). AT2 cells showed strong pink positive alkaline phosphatase staining (F), whereas the immortal cells were negative (E).
