TABLE 3.
PROTEIN IDENTIFICATIONS CONFIRMED FROM PRESENT STUDY
| Protein | Molecular Weight (D) | Accession Number | Corresponding SELDI Peak (D) | Confirmed by PMF | Confirmed by MS/MS | Confirmed By Antibody | Direction of Change in Suppuration |
|---|---|---|---|---|---|---|---|
| Calgranulin A | 10,834 | P05109 | 10,834, 10,596 | Yes | Yes | Yes | Increased |
| Calgranulin B | 12,960 | P06702 | 12,960, 13,200 | Yes | Yes | Yes | Increased |
| Calgranulin C | 10,100 | P80511 | 10,100 | Yes | Yes | No | Increased |
| Clara cell secretory protein | 7,900 | P11684 | 7,900 | Yes | Yes | Yes | Decreased |
| Proline rich salivary peptide | 8,188 | P02814 | 8,119 | No | Yes | No | Decreased |
| Lysosyme C precursor | 16,537 | P61626 | 14,600 | Yes | Yes | No | No Change |
| Cystatin s | 16,204 | P01036 | 16,079 | No | Yes | No | No Change |
| hemoglobin alpha | 15,117 | P69905 | 15,080 | No | Yes | No | No Change |
Definition of abbreviations: PMF = peptide mass fingerprinting; SELDI = surface-enhanced laser desorption/ionization.
Proteins were identified by trypsin digest and PMF and well as tandem MS/MS. When available, antibodies were used to confirm protein identification by Western blot. Molecular weight refers to the theoretical molecular weight of each protein as derived from sequence. Corresponding SELDI peak refers to the protein peak seen on SELDI analysis of sputum fluid phase (differences in molecular weight may represent post-translational modifications).