Figure 6.

CCR2-expressing fibrocytes migrate in response to CCL12. Fibrocytes were purified by magnetic separation from lung digest cultures from WT (A) or CCR2−/− mice (B). Fibrocytes were tested for chemotaxis (n = 3) to SFM, CCL12 (50 ng/ml), or fibronectin (FN) (100 μg/ml). Both genotypes were able to show significant migration in response to FN (P < 0.01 and P < 0.001, respectively). Only WT fibrocytes were able to migrate in response to CCL12 (P < 0.05). Data are representative of three similar experiments.