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Electron paramagnetic resonance (EPR) spectra of azole bound and unbound to MAC-CYP51. EPR spectra of MAC-CYP51 0.1 mM (control) or MAC-CYP51 0.1 mM bound to various azoles (3 mM each of econazole, ketoconazole, itraconazole, voriconazole, fluconazole) were obtained at a microwave frequency of 9.5 GHz (X-band) at 15K, with power and modulation of 10 mW and 1 mT. Direct binding interactions with MAC-CYP51 were noted only with econazole and ketoconazole.
