TABLE 1.

REAL-TIME SEMIQUANTITATIVE PCR^*

Primer	Control-Normalized Copy No.†	TNF-α–Normalized Copy No.†
TRPC1	78.0 ± 10.0	79.0 ± 10.0
TRPC3	2.3 ± 0.4	4.1 ± 0.7‡
CD38	0.30 ± 0.02	16.0 ± 2.0

Definition of abbreviation: TRPC, transient receptor potential subfamily C.

^*PCR was performed on first-strand cDNA generated from total RNA isolated from control or TNF-α–treated HBSMCs using primers specific for TRPC1, TRPC3, and CD38. The amplification rate was monitored using light cycler and SYBR green technologies, and data were analyzed as described in Materials and Methods. Data are expressed as copy number normalized to the house keeping gene glucose-6-phosphate dehydrogenase.

^†Per 1,000 copies.

^‡P < 0.05 compared to control.