TABLE 1.
EVALUATION OF THE QUALITY FILTER CRITERIA IN MICROARRAY CHIPS
| Name | Background < 100 | Noise < 5 | Scale Factor* | %P† | 3′/5′ β-actin < 2 | 3′/5′ GAPDH < 2 |
|---|---|---|---|---|---|---|
| 1-normoxia | 59 | 2.5 | 13.4 | 42 | 2.5 | 1.6 |
| 2-normoxia | 89 | 3.8 | 4.4 | 52 | 1.2 | 0.9 |
| 2-hyperoxia | 80 | 3.3 | 4.2 | 54 | 1.3 | 1.1 |
| 3-normoxia | 100 | 4.2 | 6.1 | 46 | 1.4 | 1,0 |
| 3-hyperoxia | 93 | 3.9 | 3.6 | 54 | 1.2 | 1,0 |
| 4-normoxia | 70 | 3.1 | 6.1 | 53 | 1.0 | 0.9 |
| 4-hyperoxia | 84 | 3.5 | 10.6 | 46 | 1.2 | 0.8 |
| 5-hyperoxia | 81 | 3.6 | 8.2 | 50 | 1.4 | 0.9 |
| Mean (70) | 82 (13) | 3.5 (0.5) | 7.1 (3.4) | 50 (4) | 1.4 (0.5) | 1 (0.2) |
Definition of abbreviation: GAPDH, glyceraldehyde phosphate deydrogenase.
*
Scaling factor of the mean intensity for each array to a set target intensity of 300.
†
Percent of genes detected as Present by the Microarray Suite 5.1 software.