TABLE 2.

PROBES FOR EMSA AND PCR PRIMERS FOR MAKING MUC7 PROMOTER MUTANTS

	Probes for EMSA	Primers for Mutation
NF-κB	wt: 5′-TTAAAAGTAAATTCCTTGAC-3′	Upper: 5′-TTAAAAGTAAATGAGTTGAC-3′
	mut: 5′-TTAAAAGTAAATGAGTTGAC-3′	Lower: 5′-GTCAACTCATTTACTTTTAA-3′
AP1	wt: 5′-TTCCTTGACACAAATGACCTTA-3′	Upper: 5′-TTCCTCAGAACAAACAGACTTA-3′
	mut: 5′-TTCCTCAGAACAAACAGACTTA-3′	Lower: 5′-TAAGTCTGTTTGTTCTGAGGAA-3′

Definition of abbreviations: AP1, activator protein-1; EMSA, electrophoretic mobility shift assay.

Mutation sites are indicated with underline. The upper-strand primer and the lower-strand primer are complementary (overlap each other).