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. 2006 Jun 22;35(6):639–650. doi: 10.1165/rcmb.2005-0325OC

Figure 7.

Figure 7.

GPX2 confers protection against oxidative damage in lung epithelial cells. (A) A549 cells were transfected with GPX2 siRNA and non targeting SS siRNA. Cells were harvested after 72 h and GPX2 message was quantified by real-time RT-PCR. (B) Inhibition of GPX2 enhances the production of lipid hydroperoxides in lung epithelial A549 cells. Knockdown of GPX2 by siRNA significantly increased the production of thiobarbutyric acid substances in tBHP-treated A549 cells. Data are mean ± SEM of three independent experiments. * Significant when compared with vehicle-treated cells; † significant when compared with SS siRNA–transfected cells. †P ⩽ 0.05. (C) Enhanced sensitivity of Nrf2 siRNA– and GPX2 siRNA–transfected A549 cells to CS condensate. Cells were exposed to CS for 48 h and viable cells were determined by MTT assay. Data are represented as percentage of viable cells relative to the vehicle-treated control. Data are means of six independent replicates, combined to generate the mean ± SD for each concentration. *P < 0.01 relative to SS siRNA.