Skip to main content
. 2006 Jul 20;35(6):714–721. doi: 10.1165/rcmb.2005-0026OC

Figure 2.

Figure 2.

Figure 2.

Figure 2.

Effect of hirudin on thrombin-induced MMP activation in HFL-1 and HBF. (A) Thrombin-induced MMP activation in HFL-1 cells. HFL-1 cells were cast into collagen gels and cultured for 5 d in the presence of cytokines and thrombin. Media were harvested for gelatin zymography. (B) Effect of hirudin on MMP activation in the presence of cytokine and thrombin in HFL-1 cells. HFL-1 cells were cast into collagen gels and cultured for 5 d in the presence or absence of cytokines, thrombin, or hirudin. Media were harvested for gelatin zymography. (C) MMP production and activation in HBFs. HBFs were cast into collagen gels and cultured for 5 d in the presence of thrombin and/or cytokines. Media were harvested for gelatin zymography. CK, cytokines (IL-1β + TNF-α); Tm, thrombin. Supernatant from HT1080 cell monolayer culture was used as a positive control.