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. 2006 Jul 20;35(6):714–721. doi: 10.1165/rcmb.2005-0026OC

Figure 4.

Figure 4.

Effect of thrombin and cytokines on TIMP-1 production. Gels containing HFL-1 fibroblasts (4.5 × 105/ml) were cast into serum- free DMEM containing thrombin (0 nM, 5 nM, 50 nM, and 500 nM) with or without cytokines (5 ng/ml TNF-α and 5 ng/ml IL-1β). After 5 d of culture, ELISA was used to identify TIMP-1 in the culture medium. Vertical axis: TIMP-1 content (% of Control). Horizontal axis: Thrombin (nM). Data presented are mean± SEM for three separate experiments, each of which included triplicate gels for each condition. *P < 0.05, **P < 0.01 compared with control.