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Thrombin directly activates MMP-2 and MMP-9. Supernatant from HT1080 cell monolayer culture was exposed to thrombin (0 nM, 5 nM, and 500 nM) for 24 h, the media were harvested, and gelatin zymography was used to identify gelatinases. Arrows indicate the latent and active forms of MMP-2 and MMP-9.
