Levels of 16 cytokines were measured simultaneously using a biometric multiplex assay from serum of mice induced with acute DSS and TNBS-induced colitis. (A) Cytokine pattern in acute colitis is represented by a macrophage-derived (TNFa, IL-6, IL-12 IFNg), strong chemotactic pattern (MIP-1a, KC), and a polarized Th1-Th17 panel (IL-17, TNFa, IFNg). (B) No significant differences were observed in levels of humoral cytokines, and other cellular cytokines and chemokines. This suggest that the cytokine profile of DSS and TNBS acute colitis is consistent with an acute inflammatory response.