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. Author manuscript; available in PMC: 2010 Mar 15.
Published in final edited form as: Anal Biochem. 2008 Dec 24;386(2):222–227. doi: 10.1016/j.ab.2008.12.008

Fig. 1.

Fig. 1

Scanned images of new slides and their stripped counterparts. (A–C) Composite images of the same area of three miRNA microarray slides hybridized with a mixture of labeled 293T RNAs and reference DNA oligonucleotides. RNA hybridization generated signals in the green channel, while reference DNA generated signals in the red channel. (D–F) Images of the same slides (A–C) after treated with 2 mM, 1 mM, or 0 mM NaOH in 0.1 x SSC. (G–I) Images of the stripped slides (D–F) after re-hybridized with the same sample of labeled 293T RNAs and reference DNAs as in (A–C). (J, K) Image of twice-stripped slides after re-hybridization. (M, N) Image of trice-stripped slides after re-hybridization. (O) Image of the slide stripped four times after re-hybridization. (P) Image of the slide stripped five times after re-hybridization.