Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
SDS-PAGE comparing the wild-type RnfG to the RnfGT175L mutant and wild-type RnfD to the RnfD-T278L mutant. Panel A: RfnG and RnfG-T175L, gel stained with Coomassie. Panel B: The same gel under UV illumination prior to staining, showing flavin fluorescence. Panel C: RfnD, RnfD-T278L, and RnfD-T187V (I, after Ni-NTA; II, after gel filtration), gel under UV illumination prior to staining, showing flavin fluorescence. Approximately 20 μg of protein was loaded in each lane.
