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(A) Visible spectra of oxidized and partially reduced RnfG. Approximately 30 μg of protein was used. Gray spectrum: oxidized (as isolated) RnfG. Black spectrum: partially reduced RnfG. The partially reduced spectrum was acquired under anaerobic conditions using dithionite as reductant. (B) Visible spectrum of oxidized RnfD wild type (black line) and the RnfD-T278L mutant (gray line). Approximately 30 μg of protein was used.
