Fig. 4. TAK1 binds H11/HspB8, but not W51C.
A. Reciprocal immunoprecipitation/immunoblotting (pull-down) of H11/HspB8 transfected Dox-treated (2 days) SKMEL2 cells using antibodies H11-181, TAK1 or preimmune (Pre) IgG. B. Pull-down of H11/HspB8-transfected A2058 cells treated with Dox (1–3 days) using TAK1 antibody. C. Pull-down of H11/HspB8-transfected [32P]-orthophosphate labeled A2058 cells with H11-181 and TAK1 antibodies and Pre IgG. D–G. Reciprocal pull-down of TAG51 (D) G361 melanoma cells (E), HEK293 (F) and NM2 (G) cells with H11-181 or TAK1 antibodies. Representative molecular weights are on the right.