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. Author manuscript; available in PMC: 2010 Mar 1.

Published in final edited form as: Int J Mol Med. 2009 Mar;23(3):389–397. doi: 10.3892/ijmm_00000143

Fig. 4 — Alanine substitution from P3-P3′ inhibits cleavage at site a but enhances that at site b. A. Six alanine (6A) were introduced to replace the 3 amino acids on either side of each cleavage site. His-tagged wild type LR and indicated mutants were synthesized by in vitro translation, purified, and digested with purified Xenopus ST3 catalytic domain. The samples were subjected to Western blotting with anti-Xenopus LR antibody. Note that all 6 lanes were run on the same gel but two lanes for a construct not relevant to this figure were removed. The figure is a representative of 2 independent experiments with similar results.

B. Quantification of the data shown in A. Shown here is the average from 2 independent experiments.

Fig. 4 — Alanine substitution from P3-P3′ inhibits cleavage at site a but enhances that at site b. A. Six alanine (6A) were introduced to replace the 3 amino acids on either side of each cleavage site. His-tagged wild type LR and indicated mutants were synthesized by in vitro translation, purified, and digested with purified Xenopus ST3 catalytic domain. The samples were subjected to Western blotting with anti-Xenopus LR antibody. Note that all 6 lanes were run on the same gel but two lanes for a construct not relevant to this figure were removed. The figure is a representative of 2 independent experiments with similar results.

B. Quantification of the data shown in A. Shown here is the average from 2 independent experiments.