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Morphological and biochemical features of neuronal differentiation in SH-SY5Y cells following treatment with retinoids. Treatment with retinoid (7 days): control (CTL), 1 μM ATRA, 1 μM 13-CRA and 0.5 μM 4-HPR. (a) Methylene blue staining to identify morphological features of neuronal differentiation. (b) Application of TRAP assay to determine any decrease in the telomerase activity. (c) Western blotting to examine levels of hTERT, N-Myc, NFP and ß-actin.
